If you have access to the equipment and materials used in the lab, please perform the lab as indicated in the manual. Chemistry of life instructor materials preparation module 3. Read this science lab report and over 89,000 other research documents. Ap biology enzyme kinetics lab report free download as word doc. Enzyme lab report leena bhamrah enzyme catalysis lab. Cover the top of the agarose container flask to minimize evaporation.
Describe how temperature, ph, enzyme concentration, andor substrate concentration can affect. Two important components of enzymes are their active sites, where the actual substrates bind, and the allosteric sites, which affect the enzyme s shape. Calculate mean, median, standard deviation, range are the 2 populations before and after selection p and f 1 actually different. Biofuel enzyme reactions kit for ap biology life science. The biofuel enzyme reactions kit for ap biology is a studentdriven, inquirybased kit that. To understand the role of guaiacol in this experiment. A substrate is a molecule that temporarily binds with the enzyme at an area on the enzyme called the active site. Ap biology investigative labs ap central the college board.
Enzyme catalysis lab report1 university of pennsylvania. Enzyme activity of turnip peroxidase investigation modified ap essential understandings completed during lab essential knowledge 2. Enzymes are a form of proteins that act as catalysts to speed up chemical reactions without being consumed by the reaction. The purpose of a biology lab report is to describe and analyze a laboratory experiment that explores a scientific concept so that other scientists might be able to replicate the experiment and investigate the principles of it.
The objective of this lab was to develop a protocol to investigate the effect of an environmental variable on the catalytic function of an enzyme. This lab fits in chapter 2 chemistry of life of freshman biology with enzyme activity. Most ap biology students earned enough pts to get a 2,3,4, but very few scored well enough on the grid ins and free response to score a 5. They act as catalysts and affect the rate of a chemical reaction. Pig dissection lab report pig dissection laboratory 10, ap biology abstract during the pig dissection lab we were determining if dissections were more beneficial than stimulations. Catalase enzymes abstract in this laboratory exercise, studies of enzyme catalase, which accelerates the breakdown of. The customary book, fiction, history, novel, scientific research. All laboratory bench tops should be wiped down with a 10% bleach solution or disinfectant to ensure cleanliness. The purpose of this lab is to investigate the impact of temperature, substrate concentration, enzyme concentration, and the presence of an inhibitor on the effectiveness and rate of an enzyme. An enzyme is a protein and is a catalyst to chemical reactions. Enzyme lab activity for ap biology and biotechnology classrooms.
Ap biology lab manual for teachers supplement lab 2. We were also dissecting a fetal pig and learning about the digestive systems, cardio, kidneys, nervous and skeletal systems. Peroxidase enzyme biology lab report half way finished. Ap biology pre lab enzyme catalysis kiara kauffman. Restriction enzyme cleavage of dna and electrophoresis ap. Enzyme lab report bio181l general biology i studocu. The amount of h 2 o 2 that decomposed at room temperature 2ml24 hours 7ml5ml2ml activity d. Americas lab report looks at a range of questions about how laboratory. It helps accelerate reactions by lowering the activation energy, which is needed for reactions in cells to progress at a higher rate. Write a balanced chemical equation with state symbols for the reaction catalyzed by peroxidase.
You will use approximately 50 ml of agarose per gel depending upon your electrophoresis apparatus. In this lab, students will use yeast as a source of catalase. We determined the rate of the enzyme catalyzed reaction. Paul andersen starts with a brief description of enzymes and substrates.
The effect of ph on the rate of enzyme catalysis of catalase objectives. Effect of ph read red book pg s158 in lab book, bullet question at top of page s158. We additionally allow variant types and after that type of the books to browse. The rate of reaction on liver, apples, and potatoes with hydrogen peroxide. The amount of h 2 o 2 decomposed is equivalent to the baseline determined in activity b minus the change in volume from table 2. Follow your teachers instructions regarding cleanup and disposal of all chemicals and materials used in this lab. Peroxidase enzyme biology lab report half way finished essay examples 635 words 3 pages how enzymes work in different environments by sarah smith biology1111 october 20, 2011 lab partner. The expectation is that this lab will be a formal writeup and then submitted. Data analysis and presentation instructor materials preparation module 2.
Data analysis and presentation data analysis and presentation. The text provides comprehensive coverage of foundational research and core biology concepts through an evolutionary lens. S153156 read peroxidase enzyme activity handout link to the left pp. These suggestions are provided to enhance the students overall lab. Ap biology enzyme lab report catalysis active site scribd. You have just read in the previous section that lactaid is really the enzyme lactase.
Peroxidase enzyme activity ap biology big idea 4, investigation an advanced inquiry lab introduction enzyme activityhow the rate of an enzyme catalyzed reaction depends on concentration, ph, temperature, metal ions, etc. The objective of this experiment is to develop an understanding of the role of restriction enzymes and agarose gel electrophoresis to cut and size dna. Enzyme activity measuring the effect of enzyme concentration. Complete the lab manual worksheets and submit your data to the moodle biology website for comparison with the work of your fellow students. Ap biology is a second year biology course that is designed to be the. Biology i laboratory manual simple book production. In this lab, we observed the role of an enzyme catalyst in conversion of hydrogen peroxide h2o2 to water and oxygen. For example, the enzyme amylase in your saliva speeds up the breakdown down of starch the substrate into simple sugars.
Purchase an ap biology test prep book for extra practice. Enzyme reaction rates using toothpickase introduction. It helps accelerate reactions by lowering the activation energy, which is. Overall this lab was relatively successful in determining the effects of enzyme concentration, substrate concentration, temperature. Ap biology grid in questions require students to use mathematics to solve biological problems. Enzymes bind temporarily to one or more of the reactants, which is called. Ap biology enzyme lab report free download as word doc. More specifically, the objective was to perform an experiment in order to. Read the background information at the beginning of the lab so you understand lactose intolerance, the action of the enzyme lactase and the commercial product, lactaid. Catalase is an enzyme that speeds up the breakdown of hydrogen peroxide into water h 2o and oxygen gas o 2. Name the substrate and products of the peroxidasecatalyzed reaction. Big genetics and information transfer 3 ap central. Enzyme catalysis overview the information will assist teachers with aspects of lab 2 that are not necessarily addressed in the lab manual.
Abstract the purpose of this lab was to test enzyme function in various environments. Enzymes bond with substrates and react with the substrates to release products. The kit includes enough materials for a class of 32 students working in groups of 4. Students will change the temperature of the substrate, hydrogen peroxide, and measure oxygen production with a gas pressure sensor. Each enzyme catalyzes one specific reaction because there is only one type of substrate molecule with the exact shape that will fit into the enzyme. If we titrate 5 time trials of a reaction of h 2 o 2 and a catalase being stopped with h 2 so 4, then we. The effects of an enzyme catalyst on h2o2 and with changed conditions introduction. He then explains how you can measure the rate of an enzyme. He then explains how you can measure the rate of an enzyme mediated reaction. Enzymes lab report inroduction in this lab we explore an enzymes activity and how it can be affected by changes to its environment. Editorial inquiries concerning this book should be directed to the college board. Ap biology enzyme kinetics lab report active site enzyme. In this lab, the enzyme catalase was used to break down hydrogen peroxide into less toxic water and oxygen gas. Download file pdf biology lab 2 enzyme catalysis answers biology lab 2 enzyme catalysis answers right here, we have countless books biology lab 2 enzyme catalysis answers and collections to check out.
Knowing a collection of facts about biology is beneficial only if you can use that information to understand and investigate a particular aspect of the natural world. Describetherelationshipbetweensubstrateconcentrationandtheinitialreactionrateofan enzyme. If the pre lab is not completed prior to the lab, then you will not receive credit. Restriction enzyme 112 cleavage of dna and electrophoresis ap biology lab 6b see page 3 for storage instructions. The results section of your lab report will include but not be limited to. Nellie greer abstract peroxidase is an enzyme found in potatoes that catalyzes the breakdown of hydrogen peroxide, h2o2, into o2 gas and water. As could be predicted, the purified enzyme from a common soil fungus has a ph optimum of 5. Substrate concentration, temperature, and ph all affect the chemical reaction.